PALS/PRISM Software Design Description (SDD): Ver. 0.51

This Software Design Description (SDD) provides detailed information on the architecture and coding for the PRISM C++ library (version 0.51). The PRISM C++ library supports consistent information sharing and in- teractions between distributed components of networked embedded systems, e.g. avionics. It is designed to reduce the complexity of the networked sys- tem by employing synchronous semantics provided by the architectural pat- tern called a Physically-Asynchronous Logically-Synchronous (PALS) system.unpublishednot peer reviewe

Adaptive Disorder Control in Data Stream Processing

Out-of-order tuples in continuous data streams may cause inaccurate query results since conventional window operators generally discard those tuples. Existing approaches use a buffer to fix disorder in stream tuples and estimate its size based on the maximum network delay seen in the streams. However, they do not provide a method to control the amount of tuples that are not saved and discarded from the buffer, although users may want to keep it within a predefined error bound according to application requirements. In this paper, we propose a method to estimate the buffer size while keeping the percentage of tuple drops within a user-specified bound. The proposed method utilizes tuples' interarrival times and their network delays for estimation, whose parameters reflect real-time stream characteristics properly. Based on two parameters, our method controls the amount of tuple drops adaptively in accordance with fluctuated stream characteristics and keeps their percentage within a given bound, which we observed through our experiments

Modeling of asymmetric giant magnetoimpedance in amorphous ribbons with a surface crystalline layer

A model describing the asymmetric giant magnetoimpedance (GMI) in field-annealed amorphous ribbons is proposed. It is assumed that the ribbon consists of an inner amorphous core and surface hard magnetic crystalline layers. The model is based on a simultaneous solution of linearizied Maxwell equations and Landau-Lifshitz equation. The coupling between the surface layers and the amorphous core is described in terms of an effective bias field. Analytical expressions for the frequency and field dependences of the ribbon impedance are found. The calculated dependences are in a qualitative agreement with results of experimental studies of the high-frequency asymmetric giant GMI in field-annealed amorphous ribbons.Comment: 13 pages, 3 figure

Magnetophoretic Decoupler for Disaggregation and Interparticle Distance Control

The manipulation of superparamagnetic beads has attracted various lab on a chip and magnetic tweezer platforms for separating, sorting, and labeling cells and bioentities, but the irreversible aggregation of beads owing to magnetic interactions has limited its actual functionality. Here, an efficient solution is developed for the disaggregation of magnetic beads and interparticle distance control with a magnetophoretic decoupler using an external rotating magnetic field. A unique magnetic potential energy distribution in the form of an asymmetric magnetic thin film around the gap is created and tuned in a controlled manner, regulated by the size ratio of the bead with a magnetic pattern. Hence, the aggregated beads are detached into single beads and transported in one direction in an array pattern. Furthermore, the simultaneous and accurate spacing control of multiple magnetic bead pairs is performed by adjusting the angle of the rotating magnetic field, which continuously changes the energy well associated with a specific shape of the magnetic patterns. This technique offers an advanced solution for the disaggregation and controlled manipulation of beads, can allow new possibilities for the enhanced functioning of lab on a chip and magnetic tweezers platforms for biological assays, intercellular interactions, and magnetic biochip systems. © 2021 The Authors. Advanced Science published by Wiley-VCH GmbH1

Performance Validation of a Planar Hall Resistance Biosensor through Beta-Amyloid Biomarker

Magnetic sensors have great potential for biomedical applications, particularly, detection of magnetically-labeled biomolecules and cells. On the basis of the advantage of the planar Hall effect sensor, which consists of improved thermal stability as compared with other magnetic sensors, we have designed a portable biosensor platform that can detect magnetic labels without applying any external magnetic field. The trilayer sensor, with a composition of Ta (5 nm)/NiFe (10 nm)/Cu (x = 0 nm~1.2 nm)/IrMn (10 nm)/Ta (5 nm), was deposited on a silicon wafer using photolithography and a sputtering system, where the optimized sensor sensitivity was 6 μV/(Oe∙mA). The detection of the magnetic label was done by comparing the signals obtained in first harmonic AC mode (1f mode) using an external magnetic field and in the second harmonic AC mode (2f mode) with a self-field generated by current passing through the sensor. In addition, a technique for the β-amyloid biomarker-based antibody-antigen sandwich model was demonstrated for the detection of a series of concentrations of magnetic labels using the self-field mode method, where the signal-to-noise ratio (SNR) was high. The generated self-field was enough to detect an immobilized magnetic tag without an additional external magnetic field. Hence, it could be possible to reduce the device size to use the point-of-care testing using a portable circuit system.1

Functionalization of Biotinylated Polyethylene Glycol on Live Magnetotactic Bacteria Carriers for Improved Stealth Properties

The early removal of drug delivery agents before reaching the affected target remains an area of interest to researchers. Several magnetotactic bacteria (MTB) have been used as self-propelled drug delivery agents, and they can also be controlled by an external magnetic field. By attaching the PEG–biotin polymer, the bacteria are turned into a stealth material that can escape from the phagocy-tosis process and reach the area of interest with the drug load. In the study, we developed a potential drug carrier by attaching the PEG–biotin to the MTB-through-NHS crosslinker to form a MTB/PEG– biotin complex. The attachment stability, efficacy, and bacterial viability upon attachment of the PEG– biotin polymer were investigated. Biological applications were carried out using a cytotoxicity assay of THP-1 cells, and the results indicate that the MTB/PEG–biotin complex is less harmful to cell viability compared to MTB alone. Along with cytotoxicity, an assay for cell association was also evaluated to assess the complex as a potential stealth material. The development of these complexes focuses on an easy, time-saving, and stable technique of polymer attachment with the bacteria, without damaging the cell’s surface, so as to make it a strong and reliable delivery agent. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.1